验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MKN-45 cells, SGC-7901 cells, NIH/3T3 cells, mouse skeletal muscle tissue, A375 cells |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human stomach cancer tissue, mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17958-1-AP targets LOX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, rabbit |
| 免疫原 | LOX fusion protein Ag12479 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lysyl oxidase |
| 别名 | Protein-lysine 6-oxidase, short form, Protein-lysine 6-oxidase, long form, Protein-lysine 6-oxidase, lysyl oxidase, EC:1.4.3.13 |
| 计算分子量 | 417 aa, 47 kDa |
| 观测分子量 | 32 kDa, 45-47 kDa |
| GenBank蛋白编号 | BC074872 |
| 基因名称 | LOX |
| Gene ID (NCBI) | 4015 |
| RRID | AB_2234541 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P28300 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LOX is a member of a multigene family (LOX, LOXL, LOXL2, LOXL3, and LOXL4). Lysyl oxidase (LOX), a copper-dependent enzyme, plays a critical role in the formation and repair of the extracellularmatrix (ECM) by catalyzing the crosslinking of elastin and collagen. LOX expression appears to be upregulated in malignancies and has recently been suggested to play a role in regulating tumor metastasis. It is synthesized as a 50 kDa proenzyme, secreted, and processed into an 32 kDa mature, active enzyme and an 18-kDa propeptide.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LOX antibody 17958-1-AP | Download protocol |
| IHC protocol for LOX antibody 17958-1-AP | Download protocol |
| IP protocol for LOX antibody 17958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Syst Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. | ||
Oncogene Stromal nicotinamide N-methyltransferase orchestrates the crosstalk between fibroblasts and tumour cells in oral squamous cell carcinoma: evidence from patient-derived assembled organoids | ||
Oncogene Cell-to-cell heterogeneity of EWSR1-FLI1 activity determines proliferation/migration choices in Ewing sarcoma cells. | ||
Int J Nanomedicine Gallbladder Cancer Progression Is Reversed by Nanomaterial-Induced Photothermal Therapy in Combination with Chemotherapy and Autophagy Inhibition. | ||
ACS Biomater Sci Eng Matrisome Provides a Supportive Microenvironment for Skin Functions of Diverse Species. | ||
