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EDG7-Specific Polyclonal antibody

EDG7-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19509-1-AP

Print datasheet

Synonyms

Edg 7, EDG7, GPCR, HOFNH30, LP A3, LPA 3, LPA receptor 3, LPA3, LPAR3, RP4 678I3

验证数据展示

  • WB analysis of PC-3 using 19509-1-AP

    WB analysis of PC-3 using 19509-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19509-1-AP (EDG7-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 19509-1-AP

    IHC staining of human heart using 19509-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19509-1-AP (EDG7-Specific antibody at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with LPAR3 antibody included
  • IHC staining of human heart using 19509-1-AP

    IHC staining of human heart using 19509-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19509-1-AP (EDG7-Specific antibody at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with LPAR3 antibody included

经过测试的应用

Positive WB detected inPC-3 cells, human testis tissue, mouse pancreas tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LPAR3 Ready-To-Use IHC Kit. LPAR3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

19509-1-AP targets EDG7-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysophosphatidic acid receptor 3
Synonyms Edg 7, EDG7, GPCR, HOFNH30, LP A3, LPA 3, LPA receptor 3, LPA3, LPAR3, RP4 678I3
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberNM_012152
Gene Symbol LPAR3
Gene ID (NCBI) 23566
RRIDAB_10641042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBY5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LPAR3, also named as EDG7 and LPA3, belongs to the G-protein coupled receptor 1 family. LPAR3 is a receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. LPAR3 may play a role in the development of ovarian cancer. Seems to be coupled to the G(i)/G(o) and G(q) families of heteromeric G proteins. The antibody is specific to LPAR3.

实验方案

Product Specific Protocols
WB protocol for EDG7-Specific antibody 19509-1-APDownload protocol
IHC protocol for EDG7-Specific antibody 19509-1-APDownload protocol
FC protocol for EDG7-Specific antibody 19509-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Comput Struct Biotechnol J

Interrelations of Sphingolipid and Lysophosphatidate Signaling with Immune System in Ovarian Cancer.

Authors - Anastasia Meshcheryakova
View Article