• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 22165-1-AP

    WB analysis using 22165-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22165-1-AP (LPAR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 22165-1-AP

    IHC staining of human ovary using 22165-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 22165-1-AP (LPAR4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 22165-1-AP

    IHC staining of human ovary using 22165-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 22165-1-AP (LPAR4 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of SW 1990 using 22165-1-AP

    IF Staining of SW 1990 using 22165-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SW 1990 cells using LPAR4 antibody (22165-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

LPAR4 Polyclonal antibody

LPAR4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22165-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

LPA4, LPA receptor 4, LPA 4, G-protein coupled receptor 23, GPR23

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inSKOV-3 cells, mouse brain tissue, HL-60 cells, 3T3-L1 cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSW 1990 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

22165-1-AP targets LPAR4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 LPAR4 fusion protein Ag17817 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lysophosphatidic acid receptor 4
别名 LPA4, LPA receptor 4, LPA 4, G-protein coupled receptor 23, GPR23
计算分子量 370 aa, 42 kDa
观测分子量 42-50 kDa
GenBank蛋白编号BC069996
基因名称 LPAR4
Gene ID (NCBI) 2846
RRIDAB_11182491
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99677
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LPAR4 antibody 22165-1-APDownload protocol
IHC protocol for LPAR4 antibody 22165-1-APDownload protocol
IF protocol for LPAR4 antibody 22165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Pancreatic cancer cells upregulate LPAR4 in response to isolation stress to promote an ECM-enriched niche and support tumour initiation

Authors - Chengsheng Wu
View Article