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LPIN1 Monoclonal antibody, PBS Only

LPIN1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G11B10

Cat no : 68124-1-PBS

Print datasheet

Synonyms

DKFZp781P1796, KIAA0188, lipin 1, LPIN1, PAP1

验证数据展示

  • WB analysis using 68124-1-Ig (same clone as 68124-1-PBS)

    WB analysis using 68124-1-Ig (same clone as 68124-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68124-1-Ig (LPIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68124-1-Ig (same clone as 68124-1-PBS)

    IHC staining of human liver cancer using 68124-1-Ig (same clone as 68124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68124-1-Ig (LPIN1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LPIN1 antibody included
  • IF Staining of NIH/3T3 using 68124-1-Ig (same clone as 68124-1-PBS)

    IF Staining of NIH/3T3 using 68124-1-Ig (same clone as 68124-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using LPIN1 antibody (68124-1-Ig, Clone: 3G11B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68124-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy LPIN1 Ready-To-Use IHC Kit. LPIN1 primary antibody included.

产品信息

68124-1-PBS targets LPIN1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen LPIN1 fusion protein Ag25813 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name lipin 1
Synonyms DKFZp781P1796, KIAA0188, lipin 1, LPIN1, PAP1
Calculated Molecular Weight 890 aa, 99 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC030537
Gene Symbol LPIN1
Gene ID (NCBI) 23175
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14693
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LPIN1, also named as KIAA0188, belongs to the lipin family.It is a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels. LPIN1 has a molecular weight of about 99 kDa but predicted bands around 130 kDa.(PMID:20231281, PMID:29765047).