验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HeLa cells, mouse skeletal muscle tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
16899-1-AP targets LPL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse |
Immunogen | LPL fusion protein Ag10431 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | lipoprotein lipase |
Synonyms | HDLCQ11, LIPD, lipoprotein lipase, LPL |
Calculated Molecular Weight | 475 aa, 53 kDa |
Observed Molecular Weight | 53 kDa |
GenBank Accession Number | BC011353 |
Gene Symbol | LPL |
Gene ID (NCBI) | 4023 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P06858 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LPL(lipoprotein lipase) is also named as LIPD and belongs to the AB hydrolase superfamily. LPL catalyses the hydrolysis of the triacylglycerol component of circulating chylomicrons and very low density lipoproteins. It plays a role in the binding of lipoprotein particles to cell-surface molecules, including sulfated glycosaminoglycans (GAGs)(PMID:21177248). The gene encodes a protein of 475 amino acids that becomes a mature protein of 448 residues after cleavage of a signal peptide. This protein is highly glycosylated and functions as a head-to-tail dimer under well-defined conditions(PMID:11154699).
发表文章
Species | Application | Title |
---|---|---|
Diabetes Rab8a Deficiency in Skeletal Muscle Causes Hyperlipidemia and Hepatosteatosis Via Impairment of Muscle Lipid Uptake and Storage. | ||
Diabetes Tissue-specific splicing and dietary interaction of a mutant As160 allele determine muscle metabolic fitness in rodents. | ||
Biology (Basel) Curcumin-Rich Diet Mitigates Non-Alcoholic Fatty Liver Disease (NAFLD) by Attenuating Fat Accumulation and Improving Insulin Sensitivity in Aged Female Mice under Nutritional Stress |