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LPO Polyclonal antibody

LPO Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10376-1-AP

Print datasheet

Synonyms

lactoperoxidase, LPO, Salivary peroxidase, SAPX, SPO

验证数据展示

  • WB analysis of human saliva using 10376-1-AP

    WB analysis of human saliva using 10376-1-AP

    human saliva were subjected to SDS PAGE followed by western blot with 10376-1-AP (LPO Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • IHC staining of human colon cancer using 10376-1-AP

    IHC staining of human colon cancer using 10376-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10376-1-AP (LPO Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 10376-1-AP

    IHC staining of human colon cancer using 10376-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10376-1-AP (LPO Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman saliva tissue, K-562 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10376-1-AP targets LPO in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lactoperoxidase
Synonyms lactoperoxidase, LPO, Salivary peroxidase, SAPX, SPO
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC107167
Gene Symbol LPO
Gene ID (NCBI) 4025
RRIDAB_2877734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22079
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LPO antibody 10376-1-APDownload protocol
IHC protocol for LPO antibody 10376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS One

Biochemical properties of thyroid peroxidase (TPO) expressed in human breast and mammary-derived cell lines.

Authors - Marlena Godlewska
View Article

PLoS One

Thyroid peroxidase (TPO) expressed in thyroid and breast tissues shows similar antigenic properties.

Authors - Marlena Godlewska
View Article
humanIHC

Front Cell Dev Biol

Identification and verification of a prognostic signature based on a miRNA-mRNA interaction pattern in colon adenocarcinoma

Authors - Qiwu Zhao
View Article
mouseWB

FASEB J

Melatonin suppresses atherosclerosis by ferroptosis inhibition via activating NRF2 pathway

Authors - Yangyang Tao
View Article