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LPP Polyclonal antibody

LPP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25045-1-AP

Print datasheet

Synonyms

Lipoma preferred partner, LPP

验证数据展示

  • WB analysis using 25045-1-AP

    WB analysis using 25045-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 25045-1-AP

    IHC staining of human small intestine using 25045-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with LPP antibody included
  • IF Staining of HeLa using 25045-1-AP

    IF Staining of HeLa using 25045-1-AP

    Immunofluorescent analysis of HeLa cells using 25045-1-AP (LPP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IP experiment of HeLa using 25045-1-AP

    IP experiment of HeLa using 25045-1-AP

    IP result of anti-LPP (IP:25045-1-AP, 4ug; Detection:25045-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • IHC staining of human small intestine using 25045-1-AP

    IHC staining of human small intestine using 25045-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with LPP antibody included

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, NIH/3T3 cells, mouse colon, mouse heart, rat heart
Positive IP detected inHeLa cells
Positive IHC detected inhuman small intestine tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LPP Ready-To-Use IHC Kit. LPP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25045-1-AP targets LPP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen LPP fusion protein Ag19205 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LIM domain containing preferred translocation partner in lipoma
Synonyms Lipoma preferred partner, LPP
Calculated Molecular Weight 612 aa, 66 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC130584
Gene Symbol LPP
Gene ID (NCBI) 4026
RRIDAB_2879869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ93052
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LPP antibody 25045-1-APDownload protocol
IHC protocol for LPP antibody 25045-1-APDownload protocol
IF protocol for LPP antibody 25045-1-APDownload protocol
IP protocol for LPP antibody 25045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Cell Death Dis

UBE2S interacting with TRIM21 mediates the K11-linked ubiquitination of LPP to promote the lymphatic metastasis of bladder cancer

Authors - Kanghua Xiao
View Article