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验证数据展示

  • WB analysis using 25045-1-AP

    WB analysis using 25045-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 25045-1-AP

    IHC staining of human small intestine using 25045-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with LPP antibody included
  • IF Staining of HeLa using 25045-1-AP

    IF Staining of HeLa using 25045-1-AP

    Immunofluorescent analysis of HeLa cells using 25045-1-AP (LPP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IP experiment of HeLa using 25045-1-AP

    IP experiment of HeLa using 25045-1-AP

    IP result of anti-LPP (IP:25045-1-AP, 4ug; Detection:25045-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • IHC staining of human small intestine using 25045-1-AP

    IHC staining of human small intestine using 25045-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with LPP antibody included

LPP Polyclonal antibody

LPP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25045-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

Lipoma preferred partner, LPP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, NIH/3T3 cells, mouse colon, mouse heart, rat heart
Positive IP detected inHeLa cells
Positive IHC detected inhuman small intestine tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LPP Ready-To-Use IHC Kit. LPP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25045-1-AP targets LPP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 LPP fusion protein Ag19205 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LIM domain containing preferred translocation partner in lipoma
别名 Lipoma preferred partner, LPP
计算分子量 612 aa, 66 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC130584
基因名称 LPP
Gene ID (NCBI) 4026
RRIDAB_2879869
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ93052
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LPP antibody 25045-1-APDownload protocol
IHC protocol for LPP antibody 25045-1-APDownload protocol
IF protocol for LPP antibody 25045-1-APDownload protocol
IP protocol for LPP antibody 25045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Cell Death Dis

UBE2S interacting with TRIM21 mediates the K11-linked ubiquitination of LPP to promote the lymphatic metastasis of bladder cancer

Authors - Kanghua Xiao
View Article