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LPPR2 Polyclonal antibody

LPPR2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25732-1-AP

Print datasheet

Synonyms

PRG 4, PLPPR2, Plasticity-related gene 4 protein, Phospholipid phosphatase-related protein type 2, Lipid phosphate phosphatase-related protein type 2

验证数据展示

  • WB analysis of 3T3-L1 using 25732-1-AP

    WB analysis of 3T3-L1 using 25732-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 25732-1-AP (LPPR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 25732-1-AP

    IHC staining of human liver cancer using 25732-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25732-1-AP (LPPR2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LPPR2 antibody included
  • IHC staining of human kidney using 25732-1-AP

    IHC staining of human kidney using 25732-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25732-1-AP (LPPR2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LPPR2 antibody included
  • IHC staining of human kidney using 25732-1-AP

    IHC staining of human kidney using 25732-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25732-1-AP (LPPR2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LPPR2 antibody included

经过测试的应用

Positive WB detected in3T3-L1 cells, HeLa cells
Positive IHC detected inhuman kidney tissue, human liver cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LPPR2 Ready-To-Use IHC Kit. LPPR2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25732-1-AP targets LPPR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen LPPR2 fusion protein Ag22839 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lipid phosphate phosphatase-related protein type 2
Synonyms PRG 4, PLPPR2, Plasticity-related gene 4 protein, Phospholipid phosphatase-related protein type 2, Lipid phosphate phosphatase-related protein type 2
Calculated Molecular Weight 427 aa, 46 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC009378
Gene Symbol LPPR2
Gene ID (NCBI) 64748
RRIDAB_2880215
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96GM1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LPPR2 antibody 25732-1-APDownload protocol
IHC protocol for LPPR2 antibody 25732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Placenta

SWATH proteomics analysis of placental tissue with intrahepatic cholestasis of pregnancy

Authors - Yuxuan Jiang
View Article