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LRCH2 Polyclonal antibody

LRCH2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24866-1-AP

Print datasheet

Synonyms

dA204F4.4, KIAA1495, LRCH2

验证数据展示

  • WB analysis of mouse brain using 24866-1-AP

    WB analysis of mouse brain using 24866-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24866-1-AP (LRCH2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 24866-1-AP

    IHC staining of human colon using 24866-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24866-1-AP (LRCH2 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, mouse ovary tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24866-1-AP targets LRCH2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen LRCH2 fusion protein Ag21701 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine-rich repeats and calponin homology (CH) domain containing 2
Synonyms dA204F4.4, KIAA1495, LRCH2
Calculated Molecular Weight 765 aa, 85 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC125224
Gene Symbol LRCH2
Gene ID (NCBI) 57631
RRIDAB_2879764
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VUJ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Leucine rich repeat and calponin homology domain containing protein 2 (LRCH2) also named as KIAA1495 is a 765 amino acid protein, which contains one CH domain and nine LRR repeats. The function of LRCH2 is still non-known.

实验方案

Product Specific Protocols
WB protocol for LRCH2 antibody 24866-1-APDownload protocol
IHC protocol for LRCH2 antibody 24866-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols