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验证数据展示

  • WB analysis using 12119-1-AP

    WB analysis using 12119-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of L02 using 12119-1-AP

    IP experiment of L02 using 12119-1-AP

    IP result of anti-LRDD (IP:12119-1-AP, 4ug; Detection:12119-1-AP 1:500) with L02 cells lysate 2800ug.

  • IHC staining of human lung using 12119-1-AP

    IHC staining of human lung using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIDD1 antibody included
  • IHC staining of human lung using 12119-1-AP

    IHC staining of human lung using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIDD1 antibody included
  • IHC staining of human lung cancer using 12119-1-AP

    IHC staining of human lung cancer using 12119-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIDD1 antibody included
  • IF Staining of HEK-293 using 12119-1-AP

    IF Staining of HEK-293 using 12119-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using LRDD antibody (12119-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

LRDD Polyclonal antibody

LRDD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12119-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

PIDD-C, PIDD1, PIDD, p53-induced death domain-containing protein 1, Leucine-rich repeat and death domain-containing protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, L02 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman lung tissue, human pancreas tissue, human liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PIDD1 Ready-To-Use IHC Kit. PIDD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12119-1-AP targets LRDD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 LRDD fusion protein Ag2761 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine-rich repeats and death domain containing
别名 PIDD-C, PIDD1, PIDD, p53-induced death domain-containing protein 1, Leucine-rich repeat and death domain-containing protein
计算分子量 893 aa, 98 kDa
观测分子量 55 kDa
GenBank蛋白编号BC014904
基因名称 LRDD
Gene ID (NCBI) 55367
RRIDAB_10694424
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HB75
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P53-induced protein with a death domain (PIDD/LRDD) is a component of the PIDDosome. PIDD contains 910 residues with 7 leucine rich repeats (LRRs), 2 ZU-5 domains and a C-terminal death domain (DD). PIDD can be cleaved into shorter fragments generating a PIDD-N fragment of 48 kD (residues 1-446), a PIDD-C fragment of 51 kD (residues 447-910) and a PIDD-CC fragment of 37 kD (residues 589-910). Auto-cleavage of PIDD determines the downstream signaling events. The PIDD-C fragment mediates activation of NFκB via the recruitment of RIP1 and NEMO, while PIDD-CC causes caspase-2 activation, which leads to apoptosis.

实验方案

Product Specific Protocols
WB protocol for LRDD antibody 12119-1-APDownload protocol
IHC protocol for LRDD antibody 12119-1-APDownload protocol
IF protocol for LRDD antibody 12119-1-APDownload protocol
IP protocol for LRDD antibody 12119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Neural Regen Res

Neat1 decreases neuronal apoptosis after oxygen and glucose deprivation.

Authors - Wei-Na Chai
View Article
mouseWB

Exp Neurol

Microglia aggravate white matter injury via C3/C3aR pathway after experimental subarachnoid hemorrhage

Authors - Lei Yang
View Article