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LRIG1 Polyclonal antibody

LRIG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22383-1-AP

Print datasheet

Synonyms

LIG 1, LIG1, LRIG1

验证数据展示

  • WB analysis of mouse liver using 22383-1-AP

    WB analysis of mouse liver using 22383-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22383-1-AP (LRIG1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • IHC staining of mouse kidney using 22383-1-AP

    IHC staining of mouse kidney using 22383-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22383-1-AP (LRIG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue, mouse skin tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22383-1-AP targets LRIG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen LRIG1 fusion protein Ag17880 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine-rich repeats and immunoglobulin-like domains 1
Synonyms LIG 1, LIG1, LRIG1
Calculated Molecular Weight 1093 aa, 119 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC071561
Gene Symbol LRIG1
Gene ID (NCBI) 26018
RRIDAB_2879095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JA1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRIG1 acts as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intracellular degradation.

实验方案

Product Specific Protocols
WB protocol for LRIG1 antibody 22383-1-APDownload protocol
IHC protocol for LRIG1 antibody 22383-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols