验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, rat liver tissue, MCF-7 cells, SKOV-3 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human lung cancer tissue, human urothelial carcinoma tissue, mouse heart tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy PTPRA Ready-To-Use IHC Kit. PTPRA primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13079-1-AP targets PTPRA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PTPRA fusion protein Ag3760 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, A |
| 别名 | LRP, HPTPalpha, HPTPA, HLPR, HEPTP |
| 计算分子量 | 793 aa, 91 kDa |
| 观测分子量 | 90-105 kDa, 140 kDa |
| GenBank蛋白编号 | BC027308 |
| 基因名称 | PTPRA |
| Gene ID (NCBI) | 5786 |
| RRID | AB_2269055 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P18433 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTPRA antibody 13079-1-AP | Download protocol |
| IHC protocol for PTPRA antibody 13079-1-AP | Download protocol |
| IF protocol for PTPRA antibody 13079-1-AP | Download protocol |
| IP protocol for PTPRA antibody 13079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncol Lett PTPRA facilitates cancer growth and migration via the TNF-α-mediated PTPRA-NF-κB pathway in MCF-7 breast cancer cells.
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Oncogene Glucose-mediated N-glycosylation of RPTPα affects its subcellular localization and Src activation | ||
Sci Signal Clustering of phosphatase RPTPα promotes Src signaling and the arthritogenic action of synovial fibroblasts | ||
EBioMedicine The circRNA circPTPRA suppresses epithelial-mesenchymal transitioning and metastasis of NSCLC cells by sponging miR-96-5p.
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