验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells |
Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30206-1-AP targets LRP1 in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | LRP1 fusion protein Ag32507 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor) |
别名 | APR, Apolipoprotein E receptor, APOER, Alpha-2-macroglobulin receptor, Alpha 2 macroglobulin receptor |
计算分子量 | 505 kDa |
观测分子量 | 600 kDa |
GenBank蛋白编号 | BC045107 |
基因名称 | LRP1 |
Gene ID (NCBI) | 4035 |
RRID | AB_3086263 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q07954 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LRP1 (Prolow-density lipoprotein receptor-related protein 1), also known as A2MR, APOER and CD91, is a type I transmembrane protein that belongs to the LDLR family. LRP1 is synthesized as a transmembrane glycosylated precursor of 600 kDa, and is subsequently cleaved to generate a large 515-kDa N-terminal extracellular subunit and an 85-kDa C-terminal transmembrane subunit, which are noncovalently associated with one another (PMID: 2112085; 8546712). LRP1 is a multifunctional receptor involved in the clearance of a large number of diverse ligands and modulating various cellular processes. LRP1 is implicated in conditions such as atherosclerosis, cancer and neurodegenerative diseases (PMID: 28381441). This antibody is raised against 20-170 aa of human LRP1.
实验方案
Product Specific Protocols | |
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WB protocol for LRP1 antibody 30206-1-AP | Download protocol |
IHC protocol for LRP1 antibody 30206-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |