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验证数据展示

  • WB analysis using 30206-1-AP

    WB analysis using 30206-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30206-1-AP (LRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30206-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30206-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30206-1-AP (LRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 30206-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30206-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30206-1-AP (LRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LRP1 Polyclonal antibody

LRP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30206-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

APR, Apolipoprotein E receptor, APOER, Alpha-2-macroglobulin receptor, Alpha 2 macroglobulin receptor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30206-1-AP targets LRP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen LRP1 fusion protein Ag32507 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor)
Synonyms APR, Apolipoprotein E receptor, APOER, Alpha-2-macroglobulin receptor, Alpha 2 macroglobulin receptor
Calculated Molecular Weight 505 kDa
Observed Molecular Weight600 kDa
GenBank Accession NumberBC045107
Gene Symbol LRP1
Gene ID (NCBI) 4035
RRIDAB_3086263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07954
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRP1 (Prolow-density lipoprotein receptor-related protein 1), also known as A2MR, APOER and CD91, is a type I transmembrane protein that belongs to the LDLR family. LRP1 is synthesized as a transmembrane glycosylated precursor of 600 kDa, and is subsequently cleaved to generate a large 515-kDa N-terminal extracellular subunit and an 85-kDa C-terminal transmembrane subunit, which are noncovalently associated with one another (PMID: 2112085; 8546712). LRP1 is a multifunctional receptor involved in the clearance of a large number of diverse ligands and modulating various cellular processes. LRP1 is implicated in conditions such as atherosclerosis, cancer and neurodegenerative diseases (PMID: 28381441). This antibody is raised against 20-170 aa of human LRP1.

实验方案

Product Specific Protocols
WB protocol for LRP1 antibody 30206-1-APDownload protocol
IHC protocol for LRP1 antibody 30206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols