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LRP2BP Polyclonal antibody

LRP2BP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25783-1-AP

Print datasheet

Synonyms

KIAA1325, LRP2 binding protein, LRP2BP, Megalin binding protein, MegBP

验证数据展示

  • WB analysis of A549 using 25783-1-AP

    WB analysis of A549 using 25783-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25783-1-AP (LRP2BP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 25783-1-AP

    IHC staining of human cerebellum using 25783-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25783-1-AP (LRP2BP antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human cerebellum using 25783-1-AP

    IHC staining of human cerebellum using 25783-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25783-1-AP (LRP2BP antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, rat lung tissue
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25783-1-AP is LRP2BP Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, rat
Immunogen LRP2BP fusion protein Ag22779 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LRP2 binding protein
Synonyms KIAA1325, LRP2 binding protein, LRP2BP, Megalin binding protein, MegBP
Calculated Molecular Weight 347 aa, 40 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC152444
Gene Symbol LRP2BP
Gene ID (NCBI) 55805
RRIDAB_2880238
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2M1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LRP2BP antibody 25783-1-APDownload protocol
IHC protocol for LRP2BP antibody 25783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols