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LRPAP1 Polyclonal antibody

LRPAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24662-1-AP

Print datasheet

Synonyms

A2MRAP, A2RAP, Alpha 2 MRAP, HBP44, LRPAP1, MRAP, RAP

验证数据展示

  • WB analysis using 24662-1-AP

    WB analysis using 24662-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24662-1-AP (LRPAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 24662-1-AP

    IHC staining of human kidney using 24662-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24662-1-AP (LRPAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPAP1 antibody included
  • IHC staining of human kidney using 24662-1-AP

    IHC staining of human kidney using 24662-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24662-1-AP (LRPAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPAP1 antibody included

经过测试的应用

Positive WB detected inHeLa cells, MDA-MB-231 cells
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LRPAP1 Ready-To-Use IHC Kit. LRPAP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24662-1-AP targets LRPAP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen LRPAP1 fusion protein Ag20304 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name low density lipoprotein receptor-related protein associated protein 1
Synonyms A2MRAP, A2RAP, Alpha 2 MRAP, HBP44, LRPAP1, MRAP, RAP
Calculated Molecular Weight 357 aa, 41 kDa
Observed Molecular Weight 39-40 kDa
GenBank Accession NumberBC112067
Gene Symbol LRPAP1
Gene ID (NCBI) 4043
RRIDAB_2879662
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LRPAP1 antibody 24662-1-APDownload protocol
IHC protocol for LRPAP1 antibody 24662-1-APDownload protocol
FC protocol for LRPAP1 antibody 24662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Stem Cell Res

Generation of a homozygous LRPAP1 knockout human embryonic stem cell line (FDCHDPe009-B) by CRISPR/Cas9 system.

Authors - Jie You
View Article