验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
10128-2-AP targets LRRC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | LRRC1 fusion protein Ag0175 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leucine rich repeat containing 1 |
| 别名 | Leucine-rich repeat-containing protein 1, LAP and no PDZ protein, LANO adapter protein, LANO |
| 计算分子量 | 59 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC003193 |
| 基因名称 | LRRC1 |
| Gene ID (NCBI) | 55227 |
| RRID | AB_2137458 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BTT6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LRRC1 consists of 524 amino acids including 16 leucine-rich repeats and a LAP-specific domain. It has been shown to be highly expressed in several human cancers and can participate in the malignant process of cancer cells, such as hepatocellular carcinoma, breast cancer and non-small cell lung cancer. (PMID: 36370237, PMID: 37505155)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LRRC1 antibody 10128-2-AP | Download protocol |
| IHC protocol for LRRC1 antibody 10128-2-AP | Download protocol |
| IF protocol for LRRC1 antibody 10128-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Mol Histol LRRC1 knockdown downregulates MACF1 to inhibit the malignant progression of acute myeloid leukemia by inactivating β-catenin/c-Myc signaling
| ||
Int J Mol Sci The Upregulation of Leucine-Rich Repeat Containing 1 Expression Activates Hepatic Stellate Cells and Promotes Liver Fibrosis by Stabilizing Phosphorylated Smad2/3
|