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  • Featured Product
  • KD/KO Validated

LRRC1 Polyclonal antibody

LRRC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10128-2-AP

Print datasheet

Synonyms

Leucine-rich repeat-containing protein 1, LAP and no PDZ protein, LANO adapter protein, LANO

验证数据展示

  • WB analysis using 10128-2-AP

    WB analysis using 10128-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10128-2-AP (LRRC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 10128-2-AP

    IHC staining of human stomach cancer using 10128-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10128-2-AP (LRRC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 10128-2-AP

    IF Staining of HEK-293 using 10128-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using LRRC1 antibody (10128-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10128-2-AP targets LRRC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen LRRC1 fusion protein Ag0175 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine rich repeat containing 1
Synonyms Leucine-rich repeat-containing protein 1, LAP and no PDZ protein, LANO adapter protein, LANO
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC003193
Gene Symbol LRRC1
Gene ID (NCBI) 55227
RRIDAB_2137458
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTT6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRRC1 consists of 524 amino acids including 16 leucine-rich repeats and a LAP-specific domain. It has been shown to be highly expressed in several human cancers and can participate in the malignant process of cancer cells, such as hepatocellular carcinoma, breast cancer and non-small cell lung cancer. (PMID: 36370237, PMID: 37505155)

实验方案

Product Specific Protocols
WB protocol for LRRC1 antibody 10128-2-APDownload protocol
IHC protocol for LRRC1 antibody 10128-2-APDownload protocol
IF protocol for LRRC1 antibody 10128-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Mol Histol

LRRC1 knockdown downregulates MACF1 to inhibit the malignant progression of acute myeloid leukemia by inactivating β-catenin/c-Myc signaling

Authors - Yao Wang
  • KD Validated
View Article
human,mouse,ratWB,IHC

Int J Mol Sci

The Upregulation of Leucine-Rich Repeat Containing 1 Expression Activates Hepatic Stellate Cells and Promotes Liver Fibrosis by Stabilizing Phosphorylated Smad2/3

Authors - Yake Wang
  • KD Validated
View Article