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LRRC41 Polyclonal antibody

LRRC41 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20457-1-AP

Print datasheet

Synonyms

Protein Muf1, PP7759, MUF1, Leucine-rich repeat-containing protein 41

验证数据展示

  • WB analysis of human brain using 20457-1-AP

    WB analysis of human brain using 20457-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20457-1-AP (LRRC41 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20457-1-AP

    IHC staining of human testis using 20457-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20457-1-AP (LRRC41 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of K-562 using 20457-1-AP

    IP experiment of K-562 using 20457-1-AP

    IP result of anti-LRRC41 (IP:20457-1-AP, 4ug; Detection:20457-1-AP 1:300) with K-562 cells lysate 1820 ug.

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20457-1-AP targets LRRC41 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LRRC41 fusion protein Ag14266 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine rich repeat containing 41
Synonyms Protein Muf1, PP7759, MUF1, Leucine-rich repeat-containing protein 41
Calculated Molecular Weight 812 aa, 87 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC004953
Gene Symbol LRRC41
Gene ID (NCBI) 10489
RRIDAB_10858385
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15345
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LRRC41 antibody 20457-1-APDownload protocol
IHC protocol for LRRC41 antibody 20457-1-APDownload protocol
IP protocol for LRRC41 antibody 20457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols