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验证数据展示

  • IHC staining of human testis using 20457-1-AP

    IHC staining of human testis using 20457-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20457-1-AP (LRRC41 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with LRRC41 antibody included
  • WB analysis using 20457-1-AP

    WB analysis using 20457-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20457-1-AP (LRRC41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 20457-1-AP

    IP experiment of K-562 using 20457-1-AP

    IP result of anti-LRRC41 (IP:20457-1-AP, 4ug; Detection:20457-1-AP 1:300) with K-562 cells lysate 1820 ug.

LRRC41 Polyclonal antibody

LRRC41 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 20457-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

Protein Muf1, PP7759, MUF1, Leucine-rich repeat-containing protein 41

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inH9C2 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LRRC41 Ready-To-Use IHC Kit. LRRC41 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20457-1-AP targets LRRC41 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LRRC41 fusion protein Ag14266 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine rich repeat containing 41
别名 Protein Muf1, PP7759, MUF1, Leucine-rich repeat-containing protein 41
计算分子量 812 aa, 87 kDa
观测分子量 80-90 kDa
GenBank蛋白编号BC004953
基因名称 LRRC41
Gene ID (NCBI) 10489
RRIDAB_10858385
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15345
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LRRC41 antibody 20457-1-APDownload protocol
IHC protocol for LRRC41 antibody 20457-1-APDownload protocol
IP protocol for LRRC41 antibody 20457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols