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  • KD/KO Validated

LRRC47 Polyclonal antibody

LRRC47 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 23217-1-AP

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Synonyms

Leucine-rich repeat-containing protein 47, KIAA1185



经过测试的应用

Positive WB detected inmouse liver tissue, L02 cells, Neuro-2a cells, NIH/3T3 cells, rat liver tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23217-1-AP targets LRRC47 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen LRRC47 fusion protein Ag19736 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine rich repeat containing 47
Synonyms Leucine-rich repeat-containing protein 47, KIAA1185
Calculated Molecular Weight 583 aa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC031301
Gene Symbol LRRC47
Gene ID (NCBI) 57470
RRIDAB_2879234
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8N1G4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.

实验方案

Product Specific Protocols
WB protocol for LRRC47 antibody 23217-1-APDownload protocol
IHC protocol for LRRC47 antibody 23217-1-APDownload protocol
IF protocol for LRRC47 antibody 23217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Structural basis for the final steps of human 40S ribosome maturation.

Authors - Michael Ameismeier
  • KD Validated
humanWB

bioRxiv

LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins

Authors - James A Saba
  • KD Validated
humanWB,CoIP,IHC

Cell Rep

The C9ORF72 repeat expansion alters neurodevelopment

Authors - Eric Hendricks