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LRRC4C Polyclonal antibody

LRRC4C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13845-1-AP

Print datasheet

Synonyms

KIAA1580, LRRC4C, Netrin G1 ligand, NGL 1, NGL1

验证数据展示

  • WB analysis of Y79 using 13845-1-AP

    WB analysis of Y79 using 13845-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13845-1-AP (LRRC4C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 13845-1-AP

    IHC staining of human gliomas using 13845-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13845-1-AP (LRRC4C antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human gliomas using 13845-1-AP

    IHC staining of human gliomas using 13845-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13845-1-AP (LRRC4C antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inY79 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

13845-1-AP targets LRRC4C in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen LRRC4C fusion protein Ag4811 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine rich repeat containing 4C
Synonyms KIAA1580, LRRC4C, Netrin G1 ligand, NGL 1, NGL1
Calculated Molecular Weight 640 aa, 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC041374
Gene Symbol LRRC4C
Gene ID (NCBI) 57689
RRIDAB_2137623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCJ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LRRC4C antibody 13845-1-APDownload protocol
IHC protocol for LRRC4C antibody 13845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Rep Med

Single-cell sequencing identifies differentiation-related markers for molecular classification and recurrence prediction of PitNET

Authors - Qilin Zhang
View Article
humanIF

Mol Med

LRRC4 functions as a neuron-protective role in experimental autoimmune encephalomyelitis.

Authors - Yan Zhang
View Article