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LRRC59 Polyclonal antibody

LRRC59 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27208-1-AP

Print datasheet

Synonyms

LRRC59, PRO1855

验证数据展示

  • WB analysis using 27208-1-AP

    WB analysis using 27208-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27208-1-AP (LRRC59 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 27208-1-AP

    IHC staining of human colon using 27208-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27208-1-AP (LRRC59 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27208-1-AP targets LRRC59 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen LRRC59 fusion protein Ag26090 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine rich repeat containing 59
Synonyms LRRC59, PRO1855
Calculated Molecular Weight 307 aa, 35 kDa
Observed Molecular Weight 35-45 kDa
GenBank Accession NumberBC052279
Gene Symbol LRRC59
Gene ID (NCBI) 55379
RRIDAB_2880801
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AG4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LRRC59 antibody 27208-1-APDownload protocol
IHC protocol for LRRC59 antibody 27208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

OrthoID: profiling dynamic proteomes through time and space using mutually orthogonal chemical tools

Authors - Ara Lee
View Article