验证数据展示
经过测试的应用
| Positive WB detected in | rat lung tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
17155-1-AP targets LRRC8A/SWELL1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | LRRC8A/SWELL1 fusion protein Ag10062 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leucine rich repeat containing 8 family, member A |
| 别名 | LRRC8A, SWELL1, LRRC8, Leucine-rich repeat-containing protein 8A, KIAA1437 |
| 计算分子量 | 810 aa, 94 kDa |
| 观测分子量 | 94 kDa |
| GenBank蛋白编号 | BC051322 |
| 基因名称 | LRRC8A |
| Gene ID (NCBI) | 56262 |
| RRID | AB_2918050 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IWT6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LRRC8A, also named as SWELL1, KIAA1437 and LRRC8, belongs to the LRRC8 family. LRRC8A is a ubiquitous and integral component of the VRAC and has been reported to be required for regulation of cell volume. It indicates that LRRC8A plays a role in regulating the flow of ions, especially anions, into and out of cells (PMID: 32079488). Additionally, there have been several reports that LRRC8A is critical for differentiation of adipocytes and lymphocytes (PMID: 31323188). The molecular weight of LRRC8A is 94 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LRRC8A/SWELL1 antibody 17155-1-AP | Download protocol |
| IHC protocol for LRRC8A/SWELL1 antibody 17155-1-AP | Download protocol |
| IP protocol for LRRC8A/SWELL1 antibody 17155-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
