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验证数据展示

  • WB analysis using 31186-1-AP

    WB analysis using 31186-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31186-1-AP (LRRFIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

LRRFIP1 Polyclonal antibody

LRRFIP1 Polyclonal Antibody for WB, ELISA
Cat No. 31186-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

GCF2, GCF 2, GC-binding factor 2, GC binding factor 2, FLIIAP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, Raji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31186-1-AP targets LRRFIP1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 LRRFIP1 fusion protein Ag34760 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine rich repeat (in FLII) interacting protein 1
别名 GCF2, GCF 2, GC-binding factor 2, GC binding factor 2, FLIIAP1
观测分子量150 kDa
GenBank蛋白编号BC108914
基因名称 LRRFIP1
Gene ID (NCBI) 9208
RRIDAB_3669888
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ32MZ4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRRFIP1 is a transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. The endogenous LRRFIP1/GCF2 proteins in several human cell lines, the one expressed from LRRFIP1/GCF2 cDNA in reticulolysates, and the recombinant LRRFIP1/GCF2 protein produced in bacteria have migrated as a band with a molecular weight (MW) of 160 kda in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

实验方案

Product Specific Protocols
WB protocol for LRRFIP1 antibody 31186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols