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验证数据展示

  • WB analysis of Neuro-2a using 27003-1-AP

    WB analysis of Neuro-2a using 27003-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27003-1-AP (LRRTM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 27003-1-AP

    IHC staining of human brain using 27003-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27003-1-AP (LRRTM3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 27003-1-AP

    IHC staining of human brain using 27003-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27003-1-AP (LRRTM3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LRRTM3 Polyclonal antibody

LRRTM3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27003-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

LRRTM3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNeuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27003-1-AP targets LRRTM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LRRTM3 fusion protein Ag25452 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine rich repeat transmembrane neuronal 3
别名 LRRTM3
计算分子量 581 aa, 66 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC111492
基因名称 LRRTM3
Gene ID (NCBI) 347731
RRIDAB_2880719
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86VH5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LRRTM3 antibody 27003-1-APDownload protocol
IHC protocol for LRRTM3 antibody 27003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols