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LRRTM3 Polyclonal antibody

LRRTM3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27003-1-AP

Print datasheet

Synonyms

LRRTM3

验证数据展示

  • WB analysis of Neuro-2a using 27003-1-AP

    WB analysis of Neuro-2a using 27003-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27003-1-AP (LRRTM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 27003-1-AP

    IHC staining of human brain using 27003-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27003-1-AP (LRRTM3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 27003-1-AP

    IHC staining of human brain using 27003-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27003-1-AP (LRRTM3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNeuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27003-1-AP targets LRRTM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LRRTM3 fusion protein Ag25452 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine rich repeat transmembrane neuronal 3
Synonyms LRRTM3
Calculated Molecular Weight 581 aa, 66 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC111492
Gene Symbol LRRTM3
Gene ID (NCBI) 347731
RRIDAB_2880719
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VH5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LRRTM3 antibody 27003-1-APDownload protocol
IHC protocol for LRRTM3 antibody 27003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols