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  • Featured Product
  • KD/KO Validated

LSG1 Polyclonal antibody

LSG1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17750-1-AP

Print datasheet

Synonyms

hLsg1, Large subunit GTPase 1 homolog, LSG1

验证数据展示

  • WB analysis of HeLa using 17750-1-AP

    WB analysis of HeLa using 17750-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17750-1-AP (LSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17750-1-AP

    IHC staining of human testis using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 17750-1-AP

    IHC staining of human testis using 17750-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17750-1-AP (LSG1 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of HeLa using 17750-1-AP

    IP experiment of HeLa using 17750-1-AP

    IP result of anti-LSG1 (IP:17750-1-AP, 4ug; Detection:17750-1-AP 1:500) with HeLa cells lysate 1400 ug.

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17750-1-AP targets LSG1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen LSG1 fusion protein Ag12016 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name large subunit GTPase 1 homolog (S. cerevisiae)
Synonyms hLsg1, Large subunit GTPase 1 homolog, LSG1
Calculated Molecular Weight 658 aa, 75 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC068500
Gene Symbol LSG1
Gene ID (NCBI) 55341
RRIDAB_2281443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H089
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LSG1 antibody 17750-1-APDownload protocol
IHC protocol for LSG1 antibody 17750-1-APDownload protocol
IP protocol for LSG1 antibody 17750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Aging Cell

Inhibition of the 60S ribosome biogenesis GTPase LSG1 causes endoplasmic reticular disruption and cellular senescence.

Authors - Asimina Pantazi
  • KD Validated
View Article
humanWB

Mol Cell Biol

The Ribosome Assembly Factor LSG1 Interacts with Vesicle-Associated Membrane Protein-Associated Proteins (VAPs)

Authors - Putri Sutjita
View Article