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LSM1 Polyclonal antibody

LSM1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10259-1-AP

Print datasheet

Synonyms

Cancer associated Sm like, CASM, LSM1, Small nuclear ribonuclear CaSm, YJL124C

验证数据展示

  • WB analysis of HeLa using 10259-1-AP

    WB analysis of HeLa using 10259-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10259-1-AP (LSM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 10259-1-AP

    IHC staining of human prostate cancer using 10259-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10259-1-AP (LSM1 antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of HeLa using 10259-1-AP

    IP experiment of HeLa using 10259-1-AP

    IP result of anti-LSM1 (IP:10259-1-AP, 4ug; Detection:10259-1-AP 1:300) with HeLa cells lysate 2000ug.

经过测试的应用

Positive WB detected inHeLa cells, mouse kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10259-1-AP targets LSM1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LSM1 fusion protein Ag0232 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Synonyms Cancer associated Sm like, CASM, LSM1, Small nuclear ribonuclear CaSm, YJL124C
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC001767
Gene Symbol LSM1
Gene ID (NCBI) 27257
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15116
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lsm1 is a component of the Lsm1-7 complex involved in cytoplasmic mRNA degradation. Lsm1 is a key subunit that determines the RNA-binding properties of this complex. The normal RNA-binding activity of this complex is crucial for mRNA decay and 3'-end protection in vivo and requires the intact Sm-domain of Lsm1. LSM1 localizes to cytoplasmic processing bodies (P bodies) involved in mRNA storage, processing, regulation, and degradation and is required for P-body formation. LSM1 proteins facilitate the formation of U4/U6 RNA duplexes in vitro and may play a role in U4/U6 snRNP formation.