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验证数据展示

  • WB analysis of HeLa using 10259-1-AP

    WB analysis of HeLa using 10259-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10259-1-AP (LSM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 10259-1-AP

    IHC staining of human prostate cancer using 10259-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10259-1-AP (LSM1 antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of HeLa using 10259-1-AP

    IP experiment of HeLa using 10259-1-AP

    IP result of anti-LSM1 (IP:10259-1-AP, 4ug; Detection:10259-1-AP 1:300) with HeLa cells lysate 2000ug.

LSM1 Polyclonal antibody

LSM1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 10259-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

Cancer associated Sm like, CASM, LSM1, Small nuclear ribonuclear CaSm, YJL124C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10259-1-AP targets LSM1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LSM1 fusion protein Ag0232 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae)
别名 Cancer associated Sm like, CASM, LSM1, Small nuclear ribonuclear CaSm, YJL124C
计算分子量 15 kDa
观测分子量 18-20 kDa
GenBank蛋白编号BC001767
基因名称 LSM1
Gene ID (NCBI) 27257
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15116
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lsm1 is a component of the Lsm1-7 complex involved in cytoplasmic mRNA degradation. Lsm1 is a key subunit that determines the RNA-binding properties of this complex. The normal RNA-binding activity of this complex is crucial for mRNA decay and 3'-end protection in vivo and requires the intact Sm-domain of Lsm1. LSM1 localizes to cytoplasmic processing bodies (P bodies) involved in mRNA storage, processing, regulation, and degradation and is required for P-body formation. LSM1 proteins facilitate the formation of U4/U6 RNA duplexes in vitro and may play a role in U4/U6 snRNP formation.