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LSM3 Polyclonal antibody

LSM3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15879-1-AP

Print datasheet

Synonyms

YLR438C, USS2, U6 snRNA-associated Sm-like protein LSm3, SMX4, MDS017

验证数据展示

  • IHC staining of mouse testis using 15879-1-AP

    IHC staining of mouse testis using 15879-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 15879-1-AP

    IHC staining of rat testis using 15879-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 15879-1-AP

    IHC staining of mouse testis using 15879-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 15879-1-AP

    IHC staining of rat testis using 15879-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 15879-1-AP

    WB analysis using 15879-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15879-1-AP (LSM3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 15879-1-AP

    IF Staining of NIH/3T3 using 15879-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using LSM3 antibody (15879-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15879-1-AP targets LSM3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LSM3 fusion protein Ag8641 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LSM3 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Synonyms YLR438C, USS2, U6 snRNA-associated Sm-like protein LSm3, SMX4, MDS017
Calculated Molecular Weight 102 aa, 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC007055
Gene Symbol LSM3
Gene ID (NCBI) 27258
RRIDAB_3669222
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62310
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LSM3, as a U6 snRNA-associated Sm-like protein, plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex)

实验方案

Product Specific Protocols
WB protocol for LSM3 antibody 15879-1-APDownload protocol
IHC protocol for LSM3 antibody 15879-1-APDownload protocol
IF protocol for LSM3 antibody 15879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols