• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

LSM3 Polyclonal antibody

LSM3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15879-1-AP

Print datasheet

Synonyms

YLR438C, USS2, U6 snRNA-associated Sm-like protein LSm3, SMX4, MDS017

验证数据展示

  • IHC staining of mouse testis using 15879-1-AP

    IHC staining of mouse testis using 15879-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 15879-1-AP

    IHC staining of rat testis using 15879-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 15879-1-AP

    IHC staining of mouse testis using 15879-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 15879-1-AP

    IHC staining of rat testis using 15879-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 15879-1-AP

    WB analysis using 15879-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15879-1-AP (LSM3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15879-1-AP targets LSM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LSM3 fusion protein Ag8641 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LSM3 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Synonyms YLR438C, USS2, U6 snRNA-associated Sm-like protein LSm3, SMX4, MDS017
Calculated Molecular Weight 102 aa, 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC007055
Gene Symbol LSM3
Gene ID (NCBI) 27258
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62310
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LSM3, as a U6 snRNA-associated Sm-like protein, plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex)

实验方案

Product Specific Protocols
WB protocol for LSM3 antibody 15879-1-APDownload protocol
IHC protocol for LSM3 antibody 15879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols