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LSM4 Polyclonal antibody

LSM4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10834-1-AP

Print datasheet

Synonyms

YER112W, U6 snRNA-associated Sm-like protein LSm4, GRP, Glycine-rich protein, Glycine rich protein

验证数据展示

  • IHC staining of human lung cancer using 10834-1-AP

    IHC staining of human lung cancer using 10834-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10834-1-AP (LSM4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LSM4 antibody included
  • WB analysis of MCF-7 using 10834-1-AP

    WB analysis of MCF-7 using 10834-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10834-1-AP (LSM4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 10834-1-AP

    IF Staining of MCF-7 using 10834-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 10834-1-AP (LSM4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF7 cells, human placenta tissue, MDA-MB-231 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy LSM4 Ready-To-Use IHC Kit. LSM4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

10834-1-AP targets LSM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen LSM4 fusion protein Ag1280 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Synonyms YER112W, U6 snRNA-associated Sm-like protein LSm4, GRP, Glycine-rich protein, Glycine rich protein
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC022198
Gene Symbol LSM4
Gene ID (NCBI) 25804
RRIDAB_2138361
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4Z0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sm-like proteins, such as LSM4, were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing

实验方案

Product Specific Protocols
WB protocol for LSM4 antibody 10834-1-APDownload protocol
IHC protocol for LSM4 antibody 10834-1-APDownload protocol
IF protocol for LSM4 antibody 10834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Rep

EDC3 phosphorylation regulates growth and invasion through controlling P-body formation and dynamics.

Authors - Jeremiah J Bearss
View Article
humanWB

Mol Cell Biol

A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis.

Authors - Berg Michael G MG
View Article