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LSM5 Polyclonal antibody

LSM5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10700-1-AP

Print datasheet

Synonyms

YER146W, U6 snRNA-associated Sm-like protein LSm5

验证数据展示

  • WB analysis of HeLa using 10700-1-AP

    WB analysis of HeLa using 10700-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10700-1-AP

    IF Staining of HeLa using 10700-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10700-1-AP (LSM5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 10700-1-AP

    WB analysis using 10700-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 10700-1-AP

    IHC staining of human liver cancer using 10700-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10700-1-AP (LSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LSM5 antibody included
  • IHC staining of human liver cancer using 10700-1-AP

    IHC staining of human liver cancer using 10700-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10700-1-AP (LSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LSM5 antibody included

经过测试的应用

Positive WB detected inHeLa cells, HL-60 cells, human brain tissue, human placenta tissue, mouse ovary tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LSM5 Ready-To-Use IHC Kit. LSM5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10700-1-AP targets LSM5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen LSM5 fusion protein Ag1145 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Synonyms YER146W, U6 snRNA-associated Sm-like protein LSm5
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC005938
Gene Symbol LSM5
Gene ID (NCBI) 23658
RRIDAB_2138475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4Y9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LSM5 antibody 10700-1-APDownload protocol
IHC protocol for LSM5 antibody 10700-1-APDownload protocol
IF protocol for LSM5 antibody 10700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols