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LSM7 Polyclonal antibody

LSM7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18941-1-AP

Print datasheet

Synonyms

LSM7, YNL147W

验证数据展示

  • WB analysis of HeLa using 18941-1-AP

    WB analysis of HeLa using 18941-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18941-1-AP (LSM7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 18941-1-AP

    IHC staining of human lymphoma using 18941-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 18941-1-AP (LSM7 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18941-1-AP targets LSM7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen LSM7 fusion protein Ag13498 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LSM7 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Synonyms LSM7, YNL147W
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC018621
Gene Symbol LSM7
Gene ID (NCBI) 51690
RRIDAB_10596483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UK45
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LSM7 antibody 18941-1-APDownload protocol
IHC protocol for LSM7 antibody 18941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cancer Res

Identification of Endogenous Adenomatous Polyposis Coli Interaction Partners and β-Catenin-Independent Targets by Proteomics.

Authors - Olesja Popow
View Article