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验证数据展示

  • WB analysis using 15593-1-AP

    WB analysis using 15593-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15593-1-AP (LSP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 15593-1-AP

    IP experiment of Raji using 15593-1-AP

    IP result of anti-LSP1 (IP:15593-1-AP, 4ug; Detection:15593-1-AP 1:300) with Raji cells lysate 4000ug.

  • IHC staining of human colon using 15593-1-AP

    IHC staining of human colon using 15593-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15593-1-AP (LSP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LSP1 Polyclonal antibody

LSP1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 15593-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA

别名

47 kDa actin binding protein, 52 kDa phosphoprotein, LSP1, lymphocyte protein 1, Lymphocyte specific protein 1, pp52, WP34

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, U-937 cells, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15593-1-AP targets LSP1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human
免疫原 LSP1 fusion protein Ag7944 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphocyte-specific protein 1
别名 47 kDa actin binding protein, 52 kDa phosphoprotein, LSP1, lymphocyte protein 1, Lymphocyte specific protein 1, pp52, WP34
计算分子量 37 kDa
观测分子量 52 kDa
GenBank蛋白编号BC001785
基因名称 LSP1
Gene ID (NCBI) 4046
RRIDAB_2250134
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP33241
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LSP1 antibody 15593-1-APDownload protocol
IHC protocol for LSP1 antibody 15593-1-APDownload protocol
IP protocol for LSP1 antibody 15593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols