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LSP1 Polyclonal antibody

LSP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15593-1-AP

Print datasheet

Synonyms

47 kDa actin binding protein, 52 kDa phosphoprotein, LSP1, lymphocyte protein 1, Lymphocyte specific protein 1, pp52, WP34

验证数据展示

  • WB analysis using 15593-1-AP

    WB analysis using 15593-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15593-1-AP (LSP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 15593-1-AP

    IP experiment of Raji using 15593-1-AP

    IP result of anti-LSP1 (IP:15593-1-AP, 4ug; Detection:15593-1-AP 1:300) with Raji cells lysate 4000ug.

  • IHC staining of human colon using 15593-1-AP

    IHC staining of human colon using 15593-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15593-1-AP (LSP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHL-60 cells, U-937 cells, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15593-1-AP targets LSP1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen LSP1 fusion protein Ag7944 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lymphocyte-specific protein 1
Synonyms 47 kDa actin binding protein, 52 kDa phosphoprotein, LSP1, lymphocyte protein 1, Lymphocyte specific protein 1, pp52, WP34
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC001785
Gene Symbol LSP1
Gene ID (NCBI) 4046
RRIDAB_2250134
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP33241
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LSP1 antibody 15593-1-APDownload protocol
IHC protocol for LSP1 antibody 15593-1-APDownload protocol
IP protocol for LSP1 antibody 15593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols