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LSS Polyclonal antibody, PBS Only

LSS Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18693-1-PBS

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Synonyms

验证数据展示

  • WB analysis of HeLa using 18693-1-AP (same clone as 18693-1-PBS)

    WB analysis of HeLa using 18693-1-AP (same clone as 18693-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18693-1-AP (LSS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 18693-1-AP (same clone as 18693-1-PBS)

    IHC staining of human liver cancer using 18693-1-AP (same clone as 18693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18693-1-AP (LSS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 18693-1-AP (same clone as 18693-1-PBS)

    IP experiment of HeLa using 18693-1-AP (same clone as 18693-1-PBS)

    IP result of anti-LSS (IP:18693-1-AP, 4ug; Detection:18693-1-AP 1:500) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 18693-1-AP (same clone as 18693-1-PBS)

    IF Staining of HepG2 using 18693-1-AP (same clone as 18693-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18693-1-AP (LSS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

产品信息

18693-1-PBS targets LSS in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LSS fusion protein Ag6955 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
Synonyms
Calculated Molecular Weight 732 aa, 83 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC035638
Gene Symbol LSS
Gene ID (NCBI) 4047
RRIDAB_2138608
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48449
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LSS, also named as OSC, is lanosterol synthase which catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. LSS gene has three isoforms with MW 74 kDa and 82-85 kDa. This antibody can recognize all these three isoforms.