LTF Monoclonal antibody
LTF Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3D1G3
验证数据展示
经过测试的应用
| Positive WB detected in | human saliva |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy LTF Ready-To-Use IHC Kit. LTF primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68509-1-Ig targets LTF in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | LTF fusion protein Ag33645 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | lactotransferrin |
| Synonyms | Lactoferrin, HLF2, Growth-inhibiting protein 12, GIG12, GIG 12 |
| Calculated Molecular Weight | 80 kDa |
| Observed Molecular Weight | 78 kDa |
| GenBank Accession Number | BC015822 |
| Gene Symbol | LTF |
| Gene ID (NCBI) | 4057 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02788 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an MW of 30 kDa and a C-terminal tryptic fragment having an MW of 50 kDa by a mild tryptic digest(PMID:3790094).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LTF antibody 68509-1-Ig | Download protocol |
| IHC protocol for LTF antibody 68509-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |