• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

LUC7L Recombinant antibody, PBS Only

LUC7L Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240311B10

Cat no : 83585-1-PBS

Print datasheet

Synonyms

Putative RNA-binding protein Luc7-like 1, LUC7L1, LUC7B1, LUC7 LIKE, hLuc7B1

验证数据展示

  • WB analysis using 83585-1-RR (same clone as 83585-1-PBS)

    WB analysis using 83585-1-RR (same clone as 83585-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83585-1-RR (LUC7L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83585-1-RR (same clone as 83585-1-PBS)

    IF Staining of HepG2 using 83585-1-RR (same clone as 83585-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LUC7L antibody (83585-1-RR, Clone: 240311B10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83585-1-RR (same clone as 83585-1-PBS)

    FC experiment of MCF-7 using 83585-1-RR (same clone as 83585-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug LUC7L Recombinant antibody (83585-1-RR, Clone:240311B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

    IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83585-1-RR (LUC7L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

    IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83585-1-RR (LUC7L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83585-1-RR

    Affinity and Kinetic Characterization of 83585-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83585-1-RR against Human LUC7L were performed. The affinity constant is 99.9 pM.

产品信息

The immunogen of 83585-1-PBS is LUC7L Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LUC7L fusion protein Ag9170 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name LUC7-like (S. cerevisiae)
Synonyms Putative RNA-binding protein Luc7-like 1, LUC7L1, LUC7B1, LUC7 LIKE, hLuc7B1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight38 kDa and 44 kDa
GenBank Accession NumberBC065198
Gene Symbol LUC7L
Gene ID (NCBI) 55692
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NQ29
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LUC7L, also known as LUC7L1, belongs to the Luc7 family. LUC7L encodes a putative RNA binding protein similar to the yeast Luc7p subunit of the U1 snRNP splicing complex, which is normally required for 5` splice site selection (PMID: 15474286). LUC7L is widely expressed. LUC7L has 3 isoforms with the molecular mass of 38, 42 and 44 kDa.