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验证数据展示

  • WB analysis of K-562 using 24202-1-AP

    WB analysis of K-562 using 24202-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24202-1-AP (LUC7L2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 24202-1-AP

    WB analysis of HeLa using 24202-1-AP

    WB result of LUC7L2 antibody (24202-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LUC7L2 transfected HeLa cells.

  • IF Staining of A431 using 24202-1-AP

    IF Staining of A431 using 24202-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using LUC7L2 antibody (24202-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  • KD/KO验证

LUC7L2 Polyclonal antibody

LUC7L2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24202-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

LUC7B2, LUC7 like 2 (S. cerevisiae), CGI-74, CGI-59, CGI 74

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24202-1-AP targets LUC7L2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 LUC7L2 fusion protein Ag21135 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LUC7-like 2 (S. cerevisiae)
别名 LUC7B2, LUC7 like 2 (S. cerevisiae), CGI-74, CGI-59, CGI 74
计算分子量 392 aa, 47 kDa
观测分子量 46 kDa
GenBank蛋白编号BC017163
基因名称 LUC7L2
Gene ID (NCBI) 51631
RRIDAB_2879455
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9Y383
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding protein LUC7L2 as a negative regulator of DNA virus-triggered innate immune response. Mechanistically, LUC7L2 directly bound to intron 3 of MITA precursor messenger RNA, inhibited its splicing, and promoted its nonsense-mediated decay, leading to its downregulation at the protein level (PMID:34155193). Moreover, LUC7L2 as a component of the U1 snRNP capable of reprogramming cellular metabolism through changes in alternative pre-mRNA splicing (PMID:33961773).

实验方案

Product Specific Protocols
WB protocol for LUC7L2 antibody 24202-1-APDownload protocol
IF protocol for LUC7L2 antibody 24202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols