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LY6H Polyclonal antibody

LY6H Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23933-1-AP

Print datasheet

Synonyms

Ly 6H, LY6H, Lymphocyte antigen 6H

验证数据展示

  • IHC staining of human testis using 23933-1-AP

    IHC staining of human testis using 23933-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23933-1-AP (LY6H Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 23933-1-AP

    IHC staining of human testis using 23933-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23933-1-AP (LY6H Antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of Raji using 23933-1-AP

    WB analysis of Raji using 23933-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 23933-1-AP (LY6H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23933-1-AP targets LY6H in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen LY6H fusion protein Ag20762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lymphocyte antigen 6 complex, locus H
Synonyms Ly 6H, LY6H, Lymphocyte antigen 6H
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC028894
Gene Symbol LY6H
Gene ID (NCBI) 4062
RRIDAB_2879366
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO94772
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LY6H is highly expressed in particular subdivisions of human brain and also in MOLT-3 and -4 acute lymphoblastic leukemia cells. It has some isoforms with MW 14-16 kDa.

实验方案

Product Specific Protocols
WB protocol for LY6H antibody 23933-1-APDownload protocol
IHC protocol for LY6H antibody 23933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols