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LY6K Polyclonal antibody

LY6K Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12026-1-AP

Print datasheet

Synonyms

Lymphocyte antigen 6K, Ly-6K, Ly 6K, HSJ001348, CO16

验证数据展示

  • WB analysis of human testis using 12026-1-AP

    WB analysis of human testis using 12026-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12026-1-AP (LY6K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 12026-1-AP

    IP experiment of PC-3 using 12026-1-AP

    IP result of anti-LY6K (IP:12026-1-AP, 4ug; Detection:12026-1-AP 1:300) with PC-3 cells lysate 3200ug.

  • IHC staining of human skin cancer using 12026-1-AP

    IHC staining of human skin cancer using 12026-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12026-1-AP (LY6K antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman testis tissue
Positive IP detected inPC-3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12026-1-AP targets LY6K in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitybovine
Immunogen LY6K fusion protein Ag2656 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lymphocyte antigen 6 complex, locus K
Synonyms Lymphocyte antigen 6K, Ly-6K, Ly 6K, HSJ001348, CO16
Calculated Molecular Weight 179 aa, 20 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC001291
Gene Symbol LY6K
Gene ID (NCBI) 54742
RRIDAB_2877817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ17RY6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LY6K antibody 12026-1-APDownload protocol
IHC protocol for LY6K antibody 12026-1-APDownload protocol
IP protocol for LY6K antibody 12026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
bovineWB

Int J Biol Macromol

TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.

Authors - Na Wang
View Article