LY96/MD2 Polyclonal antibody

LY96/MD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 11784-1-AP

Print datasheet

Synonyms

ESOP 1, ESOP1, Ly 96, LY96, LY96/MD2, lymphocyte antigen 96, MD 2, MD2, Protein MD 2



经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11784-1-AP targets LY96/MD2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen LY96/MD2 fusion protein Ag2447 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lymphocyte antigen 96
Synonyms ESOP 1, ESOP1, Ly 96, LY96, LY96/MD2, lymphocyte antigen 96, MD 2, MD2, Protein MD 2
Calculated Molecular Weight 160 aa, 18 kDa
Observed Molecular Weight20-26 kDa
GenBank Accession NumberBC020690
Gene Symbol MD2
Gene ID (NCBI) 23643
RRIDAB_2138089
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6Y9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LY96, also named as ESOP1 or MD 2, is a small secreted glycoprotein that can bind to both the hydrophobic portion of LPS and to the extracellular domain of TLR4. The interaction between MD-2 and LPS bridges the two TLR4 molecules and induces the dimerization of LPS-MD-2-TLR4, which forms the structural basis for biological functions of TLR4/MD-2 complex. Due to its essential role in mediating the interaction between LPS and TLR4, MD-2 has been extensively explored as a therapeutic target for treatment of inflammatory disorders.

实验方案

Product Specific Protocols
WB protocol for LY96/MD2 antibody 11784-1-APDownload protocol
IHC protocol for LY96/MD2 antibody 11784-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

MD2 Is a Potential Biomarker Associated with Immune Cell Infiltration in Gliomas.

Authors - Mengya Zhao
mouseWB

J Cardiovasc Transl Res

Macrophage CD36 and TLR4 Cooperation Promotes Foam Cell Formation and VSMC Migration and Proliferation Under Circadian Oscillations.

Authors - Zhen Sun
rat

Molecules

Sal B Alleviates Myocardial Ischemic Injury by Inhibiting TLR4 and the Priming Phase of NLRP3 Inflammasome.

Authors - Yang Hu
humanWB

Oncol Rep

Curcumin inhibits cell proliferation and migration in NSCLC through a synergistic effect on the TLR4/MyD88 and EGFR pathways.

Authors - Lanfeng Zhang
mouseWB,IF,IP

J Agric Food Chem

Biotransformation of 18β-Glycyrrhetinic Acid by Human Intestinal Fungus Aspergillus niger RG13B1 and the Potential Anti-Inflammatory Mechanism of Its Metabolites

Authors - Min Zhang
mouseWB,IF

Bioorg Chem

The antimicrobial peptide LK2(6)A(L) exhibits anti-inflammatory activity by binding to the myeloid differentiation 2 domain and protects against LPS-induced acute lung injury in mice

Authors - Juan Zhang