验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HL-60 cells, Jurkat cells, K-562 cells, L02 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy LYN Ready-To-Use IHC Kit. LYN primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18135-1-AP targets LYN in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | LYN fusion protein Ag12723 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | v-yes-1 Yamaguchi sarcoma viral related oncogene homolog |
| 别名 | V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, Tyrosine-protein kinase Lyn, Tyrosine protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, JTK8 |
| 计算分子量 | 512 aa, 59 kDa |
| 观测分子量 | 53-56 kDa, 66-70 kDa |
| GenBank蛋白编号 | BC075002 |
| 基因名称 | LYN |
| Gene ID (NCBI) | 4067 |
| RRID | AB_11042319 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07948 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LYN antibody 18135-1-AP | Download protocol |
| IHC protocol for LYN antibody 18135-1-AP | Download protocol |
| IF protocol for LYN antibody 18135-1-AP | Download protocol |
| IP protocol for LYN antibody 18135-1-AP | Download protocol |
| FC protocol for LYN antibody 18135-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Invest Dermatol Loss of LYN is frequent in targeted-therapy resistant melanoma cells and favors metastatic properties
| ||
