LYPLA1 Polyclonal antibody
LYPLA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse brain tissue, human brain tissue, human liver tissue, rat liver tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 6 publications below |
IHC | See 3 publications below |
产品信息
16055-1-AP targets LYPLA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | LYPLA1 fusion protein Ag9022 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | lysophospholipase I |
Synonyms | EC:3.1.2.22, EC:3.1.2.-, APT-1, APT1, APT 1 |
Calculated Molecular Weight | 230 aa, 25 kDa |
Observed Molecular Weight | 25 kDa |
GenBank Accession Number | BC010397 |
Gene Symbol | LYPLA1 |
Gene ID (NCBI) | 10434 |
RRID | AB_2234851 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O75608 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for LYPLA1 antibody 16055-1-AP | Download protocol |
IHC protocol for LYPLA1 antibody 16055-1-AP | Download protocol |
IF protocol for LYPLA1 antibody 16055-1-AP | Download protocol |
IP protocol for LYPLA1 antibody 16055-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Transl Med Identifying prognostic markers in spatially heterogeneous breast cancer microenvironment | ||
Ecotoxicol Environ Saf PM2.5 promoted lipid accumulation in macrophage via inhibiting JAK2/STAT3 signaling pathways and aggravating the inflammatory reaction. | ||
Drug Metab Dispos Identification of Bioactivating Enzymes Involved in the Hydrolysis of Laninamivir Octanoate, a Long-acting Neuraminidase Inhibitor, in Human Pulmonary Tissue. | ||
Autophagy Hypoxia-induced GPCPD1 depalmitoylation triggers mitophagy via regulating PRKN-mediated ubiquitination of VDAC1 | ||
Reprod Sci Estrogen Regulates Scribble Localization in Endometrial Epithelial Cells Through Acyl Protein Thioesterase (APT)-Mediated S-Palmitoylation in Adenomyosis |