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  • KD/KO Validated

LYPLAL1 Polyclonal antibody

LYPLAL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16146-1-AP

Print datasheet

Synonyms

KIAA1238, LYPLAL1, lysophospholipase like 1



经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16146-1-AP targets LYPLAL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen LYPLAL1 fusion protein Ag9271 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysophospholipase-like 1
Synonyms KIAA1238, LYPLAL1, lysophospholipase like 1
Calculated Molecular Weight 237 aa, 26 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberBC016711
Gene Symbol LYPLAL1
Gene ID (NCBI) 127018
RRIDAB_2138521
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VWZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lysophospholipase-like 1 protein, encoded by LYPLAL1, a 26 kDa cytosol protein which belongs to a subclass of lysophospholipase family. Human genome-wide association studies link variants of the gene encoding this enzyme to fat distribution, waist-to-hip ratio and non-alcoholic fatty liver disease. LYPLAL1 reported to be up-regulated in subcutaneous adipose tissue of obese individuals.(PMID: 25958046, PMID: 27391855, PMID: 21674055)

实验方案

Product Specific Protocols
WB protocol for LYPLAL1 antibody 16146-1-APDownload protocol
IHC protocol for LYPLAL1 antibody 16146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Chem Biol

Discovery of small-molecule enzyme activators by activity-based protein profiling.

Authors - Bernard P Kok
mouseWB

Dis Model Mech

Lyplal1 is dispensable for normal fat deposition in mice.

Authors - Rachel A Watson
  • KO Validated
human,mouseWB,IHC

Mol Cell

Targeting LYPLAL1-mediated cGAS depalmitoylation enhances the response to anti-tumor immunotherapy

Authors - Yizeng Fan
mouseWB

J Mol Endocrinol

Knockout of murine Lyplal1 confers sex-specific protection against diet-induced obesity

Authors - Rishel B Vohnoutka