验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
24313-1-AP targets LYSMD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | LYSMD3 fusion protein Ag19430 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | LysM, putative peptidoglycan-binding, domain containing 3 |
| 别名 | LYSMD3 |
| 计算分子量 | 306 aa, 35 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC136741 |
| 基因名称 | LYSMD3 |
| Gene ID (NCBI) | 116068 |
| RRID | AB_2879490 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z3D4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LYSMD3 also termed as LysM and putative peptidoglycan binding domain containing protein 3 is a 306 amino acid single-pass membrane protein that contains one LysM repeat. LysM domain encoding genes may play a role in brain and in nervous system development and functioning. LYSMD3, shows a high level of central nervous expression during embryogenesis and is also, therefore, a good candidate gene for other central nervous system (CNS) symptoms, such as psychomotor retardation, brain anomalies and muscular hypotonia of the 5q14.3 microdeletion syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LYSMD3 antibody 24313-1-AP | Download protocol |
| IHC protocol for LYSMD3 antibody 24313-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem LysMD3 is a type II membrane protein without an in vivo role in the response to a range of pathogens. | ||
Mol Biol Cell Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response. | ||