Lysozyme Polyclonal antibody

Lysozyme Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15013-1-AP

Print datasheet

Synonyms

LYZ, Muramidase, LZM, Lysozyme C, EC:3.2.1.17



经过测试的应用

Positive WB detected inTHP-1 cells, mouse lung tissue, human spleen tissue, HL-60 cells
Positive IHC detected inmouse heart tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15013-1-AP targets Lysozyme in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Lysozyme fusion protein Ag6917 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysozyme (renal amyloidosis)
Synonyms LYZ, Muramidase, LZM, Lysozyme C, EC:3.2.1.17
Calculated Molecular Weight 17 kDa
Observed Molecular Weight15-17 kDa
GenBank Accession NumberBC004147
Gene Symbol Lysozyme
Gene ID (NCBI) 4069
RRIDAB_10639507
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61626
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

实验方案

Product Specific Protocols
WB protocol for Lysozyme antibody 15013-1-APDownload protocol
IHC protocol for Lysozyme antibody 15013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cell Chem Biol

Engineered Glycocalyx Regulates Stem Cell Proliferation in Murine Crypt Organoids.

Authors - Sara H Rouhanifard
human

Arch Toxicol

Extending the viability of human precision-cut intestinal slice model for drug metabolism studies.

Authors - C Biel
mouseWB

FASEB J

Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.

Authors - Brittany R Jenkins
mouseWB

Arch Med Sci

Lysozyme promotes renal fibrosis through the JAK/STAT3 signal pathway in diabetic nephropathy

Authors - Yan Ren
mouse

Int J Mol Sci

Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays.

Authors - Wenyan Yang
ratIF

Physiol Genomics

The Atg16l1 gene: characterization of wild type, knock-in, and knock-out phenotypes in rats.

Authors - Kari Chesney