验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, PC-3 cells |
| Positive IHC detected in | human prostate hyperplasia tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15677-1-AP targets LZTS2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | LZTS2 fusion protein Ag8145 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leucine zipper, putative tumor suppressor 2 |
| 别名 | hLZTS2, KIAA1813, LAPSER1, LZTS2, Protein LAPSER1 |
| 计算分子量 | 73 kDa |
| 观测分子量 | 70-80 kDa |
| GenBank蛋白编号 | BC006212 |
| 基因名称 | LZTS2 |
| Gene ID (NCBI) | 84445 |
| RRID | AB_2878166 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BRK4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LZTS2, also named as KIAA1813 and LAPSER1, is a novel b-Catenin-interacting protein and it regulates the nuclear export of b-Catenin. LZTS2 is a negative regulator of katanin-mediated microtubule severing and release from the centrosome. It is required for central spindle formation and the completion of cytokinesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LZTS2 antibody 15677-1-AP | Download protocol |
| IHC protocol for LZTS2 antibody 15677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions. | ||
Oncogene β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.
| ||
Cancer Lett LZTS2 inhibits PI3K/AKT activation and radioresistance in nasopharyngeal carcinoma by interacting with p85.
| ||
bioRxiv α-catenin mechanosensitivity as a route to cytokinesis failure through sequestration of LZTS2
| ||
Cell Death Dis RRP15 deficiency induces ribosome stress to inhibit colorectal cancer proliferation and metastasis via LZTS2-mediated β-catenin suppression |
