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验证数据展示

  • WB analysis of rat adipose using 83655-3-RR

    WB analysis of rat adipose using 83655-3-RR

    Rat adipose tissue was subjected to SDS PAGE followed by western blot with 83655-3-RR (Leptin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 83655-3-RR

    IHC staining of human placenta using 83655-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83655-3-RR (Leptin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 83655-3-RR

    IHC staining of human placenta using 83655-3-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83655-3-RR (Leptin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83655-3-RR

    Affinity and Kinetic Characterization of 83655-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83655-3-RR against Rat Leptin were performed. The affinity constant is 3.54 nM.

Leptin Recombinant antibody

Leptin Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83655-3-RR

产品说明书

CloneNo. 240368E6

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IHC, ELISA

别名

Lep, OB, 240368E6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat adipose tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83655-3-RR targets Leptin in WB, IHC, ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 rat
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leptin
别名 Lep, OB, 240368E6
计算分子量19 kDa
观测分子量 15-16 kDa
GenBank蛋白编号NM_013076.3
基因名称 Leptin
Gene ID (NCBI) 25608
RRIDAB_3671263
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP50596
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. Leptin is the most critical hormone in the homeostatic regulation of energy balance among those so far discovered. Leptin primarily acts on the neurons of the mediobasal part of hypothalamus to regulate food intake, thermogenesis, and the blood glucose level. Leptin also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.

实验方案

Product Specific Protocols
WB protocol for Leptin antibody 83655-3-RRDownload protocol
IHC protocol for Leptin antibody 83655-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols