LightSwitch™ 3´UTR Control Vectors
LightSwitch-3-UTR-Control-Vectors-AM914
Synonyms
| Name | Format | Cat No. | Price |
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Overview
Because no single control can be ideal for all experimental conditions, Active Motif offers a panel of positive and negative control constructs for the LightSwitch™ 3´UTR Reporter Vector. These controls are used to measure background, to optimize experimental conditions and to normalize for non-specific effects associated with a treatment or change in condition. Note that the unique RenSP luciferase technology utilized in the LightSwitch Assay System eliminates the need for co-transfection of a normalizing control in most cases. So, instead of one control construct being co-transfected with every experimental plasmid, different control vectors can be used in your reporter assay experiment, each in its own well, in addition to the constructs being tested. Using the most suitable positive and negative control vectors for your specific experiment will give you the highest degree of confidence in your results.
Information on all LightSwitch 3´UTR Control Vectors is shown under the 3´UTR Controls tab below.
IMPORTANT: Because all LightSwitch reporter constructs contain the optimized RenSP luciferase gene, you MUST use our LightSwitch Luciferase Assay Reagents to obtain optimal results. (Other luciferase assay reagents are not compatible with RenSP.)
3´UTR Controls
Empty 3´UTR vector: The empty 3´UTR vector contains the RenSP luciferase reporter gene and its constitutive promoter. This construct may serve as a positive control for the transfection because of its promoter. It may also be utilized for creating additional constructs. For a vector map, annotations and primer & restriction sites, please see the Empty LightSwitch™ Reporter Vectors page.
Housekeeping gene 3´UTR vectors: Housekeeping control constructs contain the 3´UTRs for common housekeeping genes cloned downstream of the RenSP luciferase reporter gene.
Random control 3´UTR vectors: Random control constructs contain non-conserved, non-genic, and non-repetitive human genomic fragments.
Figure 1: LightSwitch assay performed using the positive and negative LightSwitch 3´UTR Controls.
Fifty ng of each 3´UTR Control Vector was transfected into HT1080 cells. Because the constitutive promoter driving the RenSP luciferase gene is the same for all vectors, it is expected that all of the vectors will give comparable signals, as this experiment does not include miRNAs to interact with the various 3´UTR regions and change the amount of luciferase produced.
The Y-axis values are shown in log scale. Absolute and relative activity will vary depending on cell line, experimental conditions and transfection reagents.
3´UTRs of housekeeping genes
| Catalog No. | Name | Description | Coordinates | Sequence |
|---|---|---|---|---|
| 32013 | LightSwitch™ ACTB 3´UTR Control |
Beta-actin | chr7–: 5340002 - 5340681 |
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| 32014 | LightSwitch™ GAPDH 3´UTR Control |
Glyceraldehyde-3-phosphate dehydrogenase |
chr12+: 6517534 - 6517833 |
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| 32015 | LightSwitch™ LDHA 3´UTR Control |
Lactose dehydrogenase A | chr11+: 18385382 - 18386064 |
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| 32016 | LightSwitch™ PPIA 3´UTR Control |
Peptidylprolyl isomerase A | chr7+: 44614226 - 44616012 |
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Random genomic fragments in 3´UTR vector
| Catalog No. | Name | Coordinates | Sequence |
|---|---|---|---|
| 32017 | LightSwitch™ Random 3´UTR Control 1 |
chr13+: 82190847 - 82193385 |
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| 32018 | LightSwitch™ Random 3´UTR Control 2 |
chr12+: 82120892 - 82121673 |
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| 32019 | LightSwitch™ Random 3´UTR Control 3 |
chr13+: 26144830 - 26145608 |
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| 32020 | LightSwitch™ Random 3´UTR Control 4 |
chr22+: 1645930 - 1646850 |
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