LightSwitch™ Promoter Control Vectors
LightSwitch-Promoter-Control-Vectors-AM913
Synonyms
| Name | Format | Cat No. | Price |
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Overview
Because no single promoter can be an ideal control for all experimental conditions, Active Motif offers a panel of positive and negative control constructs for the LightSwitch™ Promoter Reporter Vector. These controls are used to measure background, to optimize experimental conditions and to normalize for non-specific effects associated with a treatment or change in condition. Note that the unique RenSP luciferase technology utilized in the LightSwitch Assay System eliminates the need for co-transfection of a normalizing control in most cases. So, instead of one control construct being co-transfected with every experimental plasmid, different control vectors can be used in your reporter assay experiment, each in its own well, in addition to the constructs being tested. Using the most suitable positive and negative control vectors for your specific experiment will give you the highest degree of confidence in your results.
Information on all LightSwitch Promoter Control Vectors is shown under the Promoter Controls tab below.
IMPORTANT: Because all LightSwitch reporter constructs contain the optimized RenSP luciferase gene, you MUST use our LightSwitch Luciferase Assay Reagents to obtain optimal results. (Other luciferase assay reagents are not compatible with RenSP.)
Promoter Controls
Empty promoter vector: The empty promoter vector contains the RenSP luciferase reporter gene without a promoter. This construct serves as a measure of background signal in the experiment. This construct may also be used to create additional experimental constructs. For a vector map, annotations and primer & restriction sites, please see the Empty LightSwitch™ Reporter Vectors page.
Housekeeping gene promoter vectors: Housekeeping control constructs contain promoters for common housekeeping genes driving the RenSP luciferase reporter gene. These constructs serve as positive transfection controls and may also serve as controls for comparing signals between conditions if they are known to be unresponsive to the test condition.
Random control promoter vectors: Random control constructs contain 1 kb non-conserved, non-genic, non-repetitive fragments from the human genome cloned upstream of the RenSP luciferase reporter gene. These vectors produce slightly different signals than the empty promoter vector and are considered optimal negative or background controls.
Figure 1: LightSwitch assay performed using the positive and negative LightSwitch Promoter Controls.The 4 housekeeping promoter controls serve as positive controls and give relative luciferase activity. The 4 random promoter constructs and the Empty LightSwitch Reporter Vector serve as negative/background controls and establish baseline luciferase activity.
The Y-axis values are shown in log scale. Control vector activity may vary depending on cell line, treatment condition and protocol changes.
Promoters of housekeeping genes (Positive controls)
| Catalog No. | Name | Description | Coordinates | Sequence |
|---|---|---|---|---|
| 32003 | LightSwitch™ ACTB Promoter Control |
Beta-actin | chr7–: 5343428 - 5344428 |
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| 32004 | LightSwitch™ GAPDH Promoter Control |
Glyceraldehyde-3-phosphate dehydrogenase |
chr12+: 6513163 - 6514226 |
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| 32005 | LightSwitch™ LDHA Promoter Control |
Lactose dehydrogenase A | chr11+: 18372059 - 18372891 |
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| 32006 | LightSwitch™ RPL10 Promoter Control |
Ribosomal protein L10 | chrX+: 153146645 - 153147658 |
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Random genomic fragments in promoter vector (Negative controls)
| Catalog No. | Name | Coordinates | Sequence |
|---|---|---|---|
| 32007 | LightSwitch™ Random Promoter Control 1 |
chr11+: 119015626 - 119016592 |
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| 32008 | LightSwitch™ Random Promoter Control 2 |
chr1+: 226079717 - 226080649 |
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| 32009 | LightSwitch™ Random Promoter Control 3 |
chr6+: 15604416 - 15605350 |
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| 32010 | LightSwitch™ Random Promoter Control 4 |
chr16+: 1645930 - 1646850 |
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