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Lipocalin-2/NGAL Polyclonal antibody

Lipocalin-2/NGAL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31452-1-AP

Print datasheet

Synonyms

Lcn2, NGAL, Lipocalin-2, lipocalin 2

验证数据展示

  • WB analysis using 31452-1-AP

    WB analysis using 31452-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31452-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat spleen using 31452-1-AP

    IHC staining of rat spleen using 31452-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31452-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat spleen using 31452-1-AP

    IHC staining of rat spleen using 31452-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31452-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat lung tissue, rat spleen tissue, rat testis tissue
Positive IHC detected inrat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31452-1-AP targets Lipocalin-2/NGAL in WB, IHC, ELISA applications and shows reactivity with rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lipocalin 2
Synonyms Lcn2, NGAL, Lipocalin-2, lipocalin 2
Calculated Molecular Weight22kDa
Observed Molecular Weight 25 kDa
Gene Symbol Lipocalin-2/NGAL
Gene ID (NCBI) 170496
RRIDAB_3669991
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP30152
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neutrophil gelatinase-associated lipocalin (NGAL, also known as lipocalin-2, siderocalin, or LCN2) is a small molecule of almost 25 kDa that belongs to the well-defined superfamily of proteins called lipocalins. NGAL was initially found in activated neutrophils, under its role as an innate antibacterial factor. However, it subsequently was shown that many other types of cells, including in the kidney tubule, may produce NGAL in response to various injuries. NGAL may become one of the most promising next-generation biomarkers in clinical nephrology and beyond. This antibody recognizes rat Lipocalin-2/NGAL.

实验方案

Product Specific Protocols
WB protocol for Lipocalin-2/NGAL antibody 31452-1-APDownload protocol
IHC protocol for Lipocalin-2/NGAL antibody 31452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols