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  • Featured Product
  • KD/KO Validated

NGAL Recombinant antibody, PBS Only

NGAL Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G1

Cat no : 82841-1-PBS

Print datasheet

Synonyms

LCN2, lipocalin 2, Lcn 2, HNL, 2G1

验证数据展示

  • WB analysis using 82841-1-RR (same clone as 82841-1-PBS)

    WB analysis using 82841-1-RR (same clone as 82841-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82841-1-RR (Lipocalin-2/NGAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

  • WB analysis of HT-29 using 82841-1-RR (same clone as 82841-1-PBS)

    WB analysis of HT-29 using 82841-1-RR (same clone as 82841-1-PBS)

    WB result of NGAL antibody (82841-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells. This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82841-1-RR (same clone as 82841-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82841-1-RR (same clone as 82841-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82841-1-RR (NGAL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82841-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NGAL/LCN2 antibody included
  • Affinity and Kinetic Characterization of 82841-1-RR

    Affinity and Kinetic Characterization of 82841-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82841-1-RR against Human NGAL were performed. The affinity constant is 0.776 nM.

Planning an IHC experiment? We recommend our IHCeasy NGAL/LCN2 Ready-To-Use IHC Kit. NGAL/LCN2 primary antibody included.

产品信息

82841-1-PBS targets NGAL in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lipocalin 2
Synonyms LCN2, lipocalin 2, Lcn 2, HNL, 2G1
Calculated Molecular Weight23kd
Observed Molecular Weight22-25 kDa
GenBank Accession NumberNP_005555.2
Gene Symbol NGAL/LCN2
Gene ID (NCBI) 3934
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP80188
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).