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Anti-Mouse Ly-6C (HK1.4)

Ly-6C Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2c

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

HK1.4

Cat no : 65296-1-Ig

Print datasheet

Synonyms

Ly6c, Ly6c1, HK1.4, Ly 6C

验证数据展示

  • FC experiment of mouse bone marrow cells using 65296-1-Ig

    FC experiment of mouse bone marrow cells using 65296-1-Ig

    1x10^6 mouse bone marrow cells were surface stained with 0.4 ug Anti-Mouse Ly-6C (HK1.4) (65296-1-Ig, Clone: HK1.4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.4 ug Isotype Control (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse bone marrow cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65296-1-Ig targets Ly-6C in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen L3 cloned CTL cells 种属同源性预测
Host / Isotype Rat / IgG2c
Class Monoclonal
Type Antibody
Full Name lymphocyte antigen 6 complex, locus C1
Synonyms Ly6c, Ly6c1, HK1.4, Ly 6C
GenBank Accession NumberBC010764
Gene Symbol Ly-6C
Gene ID (NCBI) 17067
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Ly-6C (lymphocyte antigen 6 complex, locus C) is a 14-17 kDa GPI-linked cell surface antigen that belongs to the Ly-6 family of glycosyl phosphatidylinositol-anchored surface glycoproteins (PMID: 9192663). Ly-6C is expressed on mouse monocytes/macrophages, endothelial cells, granulocytes, thymocytes, and some T cell subsets (PMID: 2849552). The two alleles of Ly-6C, Ly-6C.1 and Ly-6C.2, result in differential expression of this protein on subpopulations of CD4+ T cells (PMID: 15057973; 9126982). Ly-6C has been reported as a marker of memory CD8+ T cells (PMID: 7543536).

实验方案

Product Specific Protocols
FC protocol for Ly-6C antibody 65296-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols