Lysozyme Monoclonal antibody
Lysozyme Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2G5A8
验证数据展示
经过测试的应用
| Positive WB detected in | human spleen tissue, RAW 264.7 cells, pig spleen tissue, rat spleen tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66456-1-Ig targets Lysozyme in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | mouse |
| Immunogen | Lysozyme fusion protein Ag6917 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | lysozyme (renal amyloidosis) |
| Synonyms | 1,4 beta N acetylmuramidase C, lysozyme, lysozyme (renal amyloidosis), Lysozyme C, LYZ, LZM |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 15-17 kDa |
| GenBank Accession Number | BC004147 |
| Gene Symbol | Lysozyme |
| Gene ID (NCBI) | 4069 |
| RRID | AB_2881825 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P61626 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Lysozyme antibody 66456-1-Ig | Download protocol |
| IHC protocol for Lysozyme antibody 66456-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Sox10 escalates vascular inflammation by mediating vascular smooth muscle cell transdifferentiation and pyroptosis in neointimal hyperplasia |
