Lysozyme Monoclonal antibody

Lysozyme Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G5A8

Cat no : 66456-1-Ig

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Synonyms

1,4 beta N acetylmuramidase C, lysozyme, lysozyme (renal amyloidosis), Lysozyme C, LYZ, LZM



经过测试的应用

Positive WB detected inhuman spleen tissue, RAW 264.7 cells, pig spleen tissue, rat spleen tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66456-1-Ig targets Lysozyme in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat, pig
Cited Reactivitymouse, rat
Immunogen Lysozyme fusion protein Ag6917 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name lysozyme (renal amyloidosis)
Synonyms 1,4 beta N acetylmuramidase C, lysozyme, lysozyme (renal amyloidosis), Lysozyme C, LYZ, LZM
Calculated Molecular Weight 17 kDa
Observed Molecular Weight15-17 kDa
GenBank Accession NumberBC004147
Gene Symbol Lysozyme
Gene ID (NCBI) 4069
RRIDAB_2881825
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61626
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

实验方案

Product Specific Protocols
WB protocol for Lysozyme antibody 66456-1-IgDownload protocol
IHC protocol for Lysozyme antibody 66456-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Rep

Sox10 escalates vascular inflammation by mediating vascular smooth muscle cell transdifferentiation and pyroptosis in neointimal hyperplasia

Authors - Xin Xu
ratIHC

J Pharmacol Sci

Oxymatrine alleviates NSAID-associated small bowel mucosal injury by regulating MIP-1/CCR1 signalling and gut microbiota

Authors - Ming Chen